Sera Tr

نویسندگان

  • Leslie Rubin
  • Patrick Durkin
  • Gary Diamond
چکیده

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Identification of delta/notch-like epidermal growth factor-related receptor as the Tr antigen in paraneoplastic cerebellar degeneration.

OBJECTIVE Anti-Tr is among the better described autoantibodies in paraneoplastic cerebellar degeneration (PCD) combined with Hodgkin lymphoma (HL); however, the Tr antigen remains unidentified. METHODS We used immunoprecipitation of total rat brain extract followed by mass spectrometry to identify the antigen recognized by anti-Tr-positive sera. By Western blotting and cell-based assays, we t...

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Purification of TR-b, a Reiter treponeme protein antigen precipitating with antibodies in human syphilitic sera.

TR-b is a Reiter treponeme antigen, cross-reacting with an antigen in Treponema pallidum (Nichols pathogenic strain). Sera from patients with secondary syphilis contain precipitating antibodies against TR-b. The isolation of TR-b from a bacterial sonic extract is described here. It involved five fractionation steps: anion-exchange chromatography (DE-52 Whatman), gel filtration (Ac-A-22 Ultrogel...

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Cloning, characterization, and serodiagnostic evaluation of Leishmania infantum tandem repeat proteins.

Visceral leishmaniasis (VL) is a form of leishmaniasis, which is caused by infection with the protozoan parasite Leishmania, and is often fatal unless it is treated. Rapid and accurate diagnosis of VL is important for effective treatment. Here we report the cloning of previously undescribed tandem repeat (TR) proteins of Leishmania infantum and an evaluation of VL patient antibody responses to ...

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The growth-promoting effect of bacterial iron for serum-exposed bacteria.

Bacterial ability to obtain iron in bovine serum or in media containing transferrin (Tr) or conalbumin (Ca) was investigated by using serum-resistant (virulent) and serum-sensitive (avirulent) strains of Escherichia coli and Salmonella typhimurium. Bacteria growing in bovine serum enriched with radioactive iron-saturated Tr or with radioactive iron-saturated enterobactin (E) did not acquire rad...

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تاریخ انتشار 2002